Pronulife Lvucare
PRONULIFE LvUCare is a defined three strain probiotic blend developed for uric acid management and liver support, sold as a freeze dried powder concentrate. The formula combines Limosilactobacillus fermentum TSF331, Limosilactobacillus reuteri TSR332 and Lactiplantibacillus plantarum TSP05, strains selected for their capacity to degrade purine derived compounds in the digestive tract and studied in the context of fatty liver and elevated uric acid.
The material is a cream to light beige free flowing powder with the faint fermented odour characteristic of freeze dried lactic cultures. Its strength is expressed as colony forming units per gram rather than as a chemical assay, with a reference concentrate of around 100 billion CFU per gram diluted with maltodextrin or microcrystalline cellulose to the potency the finished dose form calls for.
Viability rather than solubility decides how the powder is handled. The dried cells are hygroscopic, and moisture uptake is the main route by which viable count is lost, ahead of oxygen and heat. The powder is dosed under low humidity, packed under nitrogen in aluminium foil laminate and held at or below 4 degrees Celsius, with a shelf life of about 24 months under those conditions. It disperses in cold water but is added only after any thermal step, since pasteurisation inactivates the culture.
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- Function
- Probiotic
Specifications
| Item | Specification |
|---|---|
| Appearance | Cream to light beige free flowing powder |
| Odour | Characteristic, faintly fermented |
| Viable Count | Conforms to declared potency, reference grade 100 billion CFU/g |
| Strain Composition | L. fermentum TSF331, L. reuteri TSR332, L. plantarum TSP05 |
| Identification | 16S rRNA sequencing to species, pulsed field gel electrophoresis to strain |
| Loss on Drying | ≤ 5.0% |
| Water Activity | ≤ 0.20 |
| pH, 10% suspension | 4.0 to 6.5 |
| Lead | ≤ 1.0 mg/kg |
| Arsenic | ≤ 1.0 mg/kg |
| Total Plate Count, non probiotic | ≤ 1,000 CFU/g |
| Yeast and Mould | ≤ 100 CFU/g |
| E. coli | Absent in 1 g |
| Salmonella | Absent in 25 g |
| Storage and Shelf Life | 24 months at ≤ 4°C, sealed under nitrogen |
Common Markets Grades
The blend is traded as a standardised freeze dried powder declared at a fixed count per gram at time of manufacture, with a deliberate overage so the label claim still holds at the end of shelf life. The reference specification is around 100 billion CFU per gram, and lower strengths are the same fermentation biomass cut with carrier rather than a separate product.
Particle size is controlled so the powder blends uniformly with excipients and does not segregate during capsule or sachet filling, and an agglomerated grade is offered where dusting on high speed lines is a concern. Microencapsulated versions with a lipid or protein coating are produced for matrices carrying meaningful water activity or where gastric survival is written into the specification. Non-GMO, gluten free, kosher and halal declarations accompany the standard trade grades.
Applications
- Liver support and metabolic health capsules and tablets, blended dry with excipients under controlled humidity
- Uric acid management supplements, often formulated alongside plant extracts such as chicory or celery seed
- Single dose stick packs and sachets, which protect viability behind an individual foil barrier without needing a cold chain in trade
- Hangover and after drinking formats, positioned around social drinking occasions and late nights
- Powdered drink mixes and effervescent formats, where the culture is added to the dry blend rather than to the liquid
- Combination products with B vitamins, amino acids or milk thistle extract for liver positioning
- Ready to drink shot bottles held under refrigeration, dosed after any heat treatment
- Synbiotic formulations with prebiotic fibres such as inulin or fructo-oligosaccharides, blended dry immediately before filling
Regulatory Status
In the United States the species in the blend are used in conventional foods on GRAS conclusions and are marketed as dietary ingredients under the dietary supplement framework. Statements about liver function or uric acid are structure and function claims subject to supplement labelling rules rather than authorised health claims, and any language suggesting treatment of gout or liver disease would move the product into the drug category. Strain designation and declared viable count are the main label obligations.
In the European Union live cultures of this kind are food ingredients rather than additives, so no E number applies. Limosilactobacillus fermentum, Limosilactobacillus reuteri and Lactiplantibacillus plantarum all hold qualified presumption of safety status at species level in the European Food Safety Authority framework, while the term probiotic itself remains restricted on labels in most member states because no health claim for these cultures carries EU authorisation. The joint FAO and WHO guidelines on the evaluation of probiotics in food set the accepted international requirements for strain identification, viability declaration and labelling, and Codex has no strain specific standard covering the blend.
Manufacturing Process
Each of the three strains is grown separately, since their optimum temperature, oxygen tolerance and medium requirements differ. A working seed vial is expanded through flask and seed fermenter stages into a production fermenter charged with a fermentable carbohydrate, a nitrogen source such as yeast extract or a dairy free peptone, and mineral salts. The vessels are run microaerophilic with pH held near neutral by automatic alkali dosing, so that the lactic acid the cultures generate does not stop growth before the target cell density is reached.
At harvest each broth is concentrated by continuous centrifugation or by membrane filtration to a thick cell cream, then blended with cryoprotectants, typically sucrose, trehalose and a protein or hydrocolloid, to stabilise the cell membranes through freezing. The cream is pelletised in liquid nitrogen or frozen on trays and freeze dried until residual moisture falls to the low single figures. Each dried biomass is milled and assayed for viable count and strain identity, and the three are then blended with carrier to the declared ratio and potency and packed under nitrogen in foil laminate.
