CAS 99-20-7

Trehalose

Trehalose is a non-reducing disaccharide composed of two D-glucose units linked α,α-1,1 through their anomeric carbons.

The CAS number is 99-20-7 and the molecular formula is C₁₂H₂₂O₁₁. The unique α,α-1,1 linkage makes the molecule unusually stable to heat, acid, and the Maillard reaction.

Trehalose is about 45 percent as sweet as sucrose, dissolves cleanly without aftertaste, and acts as a powerful cryoprotectant and protein stabiliser in food, pharmaceutical, and biotechnology applications.

We source bulk Trehalose from top China manufacturers for our partners, working with several of them. The manufacturers are in Shandong, Hebei and Jiangsu. A smaller requirement can ship in a combined container with other ingredients.

Please send the grade you need, the volume and your destination port, and we will come back with a solution and a price.

or call +86-21-6858 0751

Packaging: 25 kg kraft paper bag with PE inner liner

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HACCP
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Specifications

Grades / Types Available: Food Grade

Item	Specification
Appearance	White crystalline powder
Assay (dry basis)	≥ 98.0%
Moisture	≤ 2.0%
pH (10% solution)	4.5 - 7.5
Specific Rotation	+196° to +202°
Heavy Metals (as Pb)	≤ 5 mg/kg
Arsenic	≤ 1 mg/kg
Reducing Sugars	≤ 0.5%
Sulphated Ash	≤ 0.1%
Purity	99%
Ash	0.05%
Loss on Drying	1.5%
Total Plate Count	<300 cfu/g
Yeast & Mold	<100 cfu/g
Melting Point	97-101°C (decomposition)
Water Solubility	High (68.9 g/100 mL at 20°C)
GMO Status	Non-GMO
Allergen Status	Allergen free
Bulk Density	~0.65 g/mL
Solid Content	>98%
Taste	Mildly sweet
Color	White
Smell	Odorless

Common Markets Grades

Food, pharmaceutical and high-purity research grades of trehalose all use the dihydrate crystal.

Food grade meets the JECFA and Codex specifications and comes as a free-flowing white crystal or fine powder.

Pharmaceutical grade meets USP-NF, Ph. Eur., and JP monographs and is used in lyophilized injectable formulations and oral solid dose.

Cell-culture and bioprocess grade carries tight endotoxin limits and low residual metal content for biopharmaceutical use.

An anhydrous trehalose grade is produced for moisture-sensitive applications, although the dihydrate is the standard form.

Applications

Freezing and freeze-drying protectant for protein-based pharmaceuticals and vaccines
Stabiliser in monoclonal antibody and enzyme formulations
Texture and moisture retention in bakery, confectionery, and rice products
Cryoprotectant in frozen seafood, meat, and noodle products
Flavour stabiliser and Maillard-suppressor in retort and UHT processed foods
Sweetener and bulking agent in beverages, jellies, and dairy desserts
Stabiliser in cosmetic formulations including anhydrous balms and serums
Cell preservation media for blood platelets and stem cells
Embryo and sperm cryopreservation in reproductive medicine
Oral care products targeting dry mouth and tooth-friendly sweetening
Carrier in spray-dried nutraceutical and probiotic formulations

Brands Using Trehalose

Widely-distributed consumer products that list Trehalose on the ingredient label.

Brand names, product names and logos are the property of their owners. They appear here as published evidence that the ingredient is used, taken from the brand's own label or website. Their appearance is not an endorsement, and it does not mean the brand buys from us or from any manufacturer listed on this site.

China Manufacturers of Trehalose

Top Trehalose manufacturers in China.

Company names and logos are the property of the manufacturers themselves. They are listed here because our own directory records them as producing this ingredient. A listing is not an endorsement by them, and we do not represent them or speak on their behalf.

How We Help Source Trehalose

Direct Factory Sourcing
We run the whole process: selecting the manufacturer from the top Chinese food ingredient producers, negotiating the price, verifying quality, and coordinating the logistics. You deal with us instead of with several factories at once.
Original Packaging and Documents from the Manufacturer
The manufacturer sends your Trehalose out of its own factory in the original packing, through to your destination port. The COA, COO, MSDS, TDS and any certifications reach you from the manufacturer as well.
Free Samples and Lead Time
We send 100 to 500 g free for your own laboratory to test and you cover only the courier, usually within 2 to 3 working days. ETD is usually within 7 to 15 days once the order is confirmed, and we check that date against the factory's own manufacturing schedule before we give it to you.
Combined Shipment
We can load Trehalose together with other ingredients such as Fructooligosaccharides, Dextrose and Dextrose Monohydrate into one 20' FCL. If your quantity is small, we can also ship LCL.

Regulatory Status

Trehalose is approved as a food ingredient in the European Union, United States, Japan, China, and most other major markets.

The U.S. FDA granted GRAS status under GRN 000045 in 2000. The EU authorised it as a novel food in 2001.

JECFA reviewed trehalose and concluded no specified ADI was required on safety grounds.

Pharmacopoeial monographs appear in the USP-NF, European Pharmacopoeia, and Japanese Pharmacopoeia.

Manufacturing Process

Trehalose Manufacturing Process

Loading process diagram…

Modern trehalose production uses an enzymatic process on starch developed by Hayashibara of Japan in the 1990s.

Liquefied starch is treated sequentially with maltooligosyl trehalose synthase (MTSase) and maltooligosyl trehalose trehalohydrolase (MTHase). MTSase converts the terminal maltooligosaccharide α-1,4 linkage to the α,α-1,1 trehalose linkage, and MTHase cleaves the trehalose unit from the remaining chain.

The reaction mass is treated with glucoamylase to convert residual oligosaccharides to glucose, which is then separated by chromatography.

The purified trehalose solution is decolorised, demineralized, concentrated, and crystallised as the dihydrate.