Bifidobacterium longum subsp. longum BL300
Bifidobacterium longum subsp. longum BL300 is a single strain probiotic culture sold as a freeze dried powder concentrate for supplements, dairy and infant and adult nutrition. B. longum subsp. longum is one of the three subspecies of Bifidobacterium longum and is a dominant member of the adult colonic microbiota, a Gram positive, non spore forming, strictly anaerobic rod that ferments carbohydrate by the fructose-6-phosphate phosphoketolase pathway to acetate and lactate.
The trade material is a cream to light beige free flowing powder with a faint fermented odour. Its strength is declared as colony forming units per gram rather than as a chemical assay, and the fermentation biomass is diluted with maltodextrin or microcrystalline cellulose to the potency the finished dose form requires.
Because the organism is a strict anaerobe, oxygen exposure limits shelf life as much as moisture and temperature do. The powder is hygroscopic, is dosed under low humidity, and is packed under nitrogen in aluminium foil laminate and stored refrigerated or frozen. It disperses in cold water but cannot be carried through pasteurisation, extrusion or spray drying, so it is added after every thermal step in a process.
We source bulk Bifidobacterium longum subsp. longum BL300 from top China manufacturers for our partners. The manufacturer is in Jiangsu. A smaller requirement can ship in a combined container with other ingredients.
Please send the grade you need, the volume and your destination port, and we will come back with a solution and a price.
or call +86-21-6858 0751
- Function
- Probiotic
Specifications
| Item | Specification |
|---|---|
| Appearance | Cream to light beige free flowing powder |
| Odour | Characteristic, faintly fermented |
| Viable Count | Conforms to declared potency, typically 10 to 300 billion CFU/g |
| Identification | Bifidobacterium longum subsp. longum by 16S rRNA sequencing |
| Strain Confirmation | Pulsed field gel electrophoresis against reference pattern |
| Loss on Drying | ≤ 5.0% |
| Water Activity | ≤ 0.20 |
| pH (10% suspension) | 4.0 to 6.5 |
| Lead | ≤ 1.0 mg/kg |
| Arsenic | ≤ 1.0 mg/kg |
| Total Plate Count, non probiotic | ≤ 1,000 CFU/g |
| Yeast and Mould | ≤ 100 CFU/g |
| E. coli | Absent in 1 g |
| Salmonella | Absent in 25 g |
| Staphylococcus aureus | Absent in 1 g |
Common Markets Grades
The strain is traded as a standardised freeze dried powder declared at a fixed viable count per gram at time of manufacture, with a deliberate overage so that the labelled count still holds at the end of shelf life. Concentrations of roughly 10, 50, 100 and 300 billion CFU per gram are the usual trade points, all cut from the same biomass and differing only in the proportion of carrier.
Beyond the plain powder, a microencapsulated grade with a lipid or protein coating is produced for matrices with meaningful water activity and for formats where gastric survival is specified, and an agglomerated grade is offered where dusting and segregation on high speed filling lines are a concern. A heat inactivated, or postbiotic, version of the same biomass is also traded where live cell viability is not required. Non-GMO, gluten free, kosher and halal declarations accompany the standard grades.
Applications
- Adult digestive health capsules and tablets, blended dry with excipients under controlled humidity
- Single dose stick packs and sachets, which hold viability behind an individual foil barrier without a cold chain in trade
- Infant and follow on formula, added into the dry blend after spray drying so the culture never meets the drier
- Paediatric drops suspended in a refined oil, a water free carrier that keeps the cells dormant
- Fermented dairy and drinking yoghurt, dosed after heat treatment as an adjunct to the acidifying starter
- Plant based drinks and fermented plant bases, where the strain carries the live culture declaration
- Synbiotic combinations with galacto-oligosaccharides or inulin, which the organism ferments preferentially
- Medical and clinical nutrition powders, where a single characterised strain simplifies the substantiation file
- Chewable tablets and gummies, requiring a coated grade to survive compression and water activity
China Manufacturers of Bifidobacterium longum subsp. longum BL300
Top Bifidobacterium longum subsp. longum BL300 manufacturers in China.
Company names and logos are the property of the manufacturers themselves. They are listed here because our own directory records them as producing this ingredient. A listing is not an endorsement by them, and we do not represent them or speak on their behalf.
Regulatory Status
In the United States Bifidobacterium longum is used in conventional foods on a GRAS basis, with notified GRAS conclusions covering specific strains including use in infant formula, and is marketed as a dietary ingredient under the dietary supplement framework. Digestive or immune statements are structure and function claims subject to supplement labelling rules rather than authorised health claims, and the main label obligations are the strain designation and the declared viable count.
In the European Union live cultures of this kind are food ingredients rather than additives, so no E number applies. Bifidobacterium longum holds qualified presumption of safety status at species level in the EFSA framework, while most member states restrict the term probiotic on labels because no health claim for these cultures carries authorisation. The joint FAO and WHO guidelines on the evaluation of probiotics in food set the internationally recognised requirements for strain identification, viability declaration and labelling, and Codex has no strain specific standard for the organism.
Manufacturing Process
The strain is grown by submerged anaerobic batch fermentation. A working seed vial is expanded through flask and seed fermenter stages into a production vessel charged with a fermentable carbohydrate, a nitrogen source such as yeast extract or a dairy free peptone, mineral salts and a reducing agent to hold the redox potential low enough for a strict anaerobe. The headspace is blanketed with nitrogen or carbon dioxide throughout, and pH is held near neutral by automatic alkali dosing, since the acetate and lactate the culture produces would otherwise stop growth well before the target cell density is reached.
At harvest the broth is concentrated by continuous centrifugation or by membrane filtration to a thick cell cream. Cryoprotectants, typically sucrose, trehalose and a protein or hydrocolloid, are blended in to stabilise the cell membranes through freezing. The cream is pelletised in liquid nitrogen or frozen on trays and freeze dried until residual moisture falls to the low single figures. The dried cake is milled, assayed for viable count and strain identity, blended with carrier to the declared potency and packed under nitrogen.
